RSV PCR Controls AffiCHECK are designed to provide quality control for RSV PCR assays. These controls help ensure the accuracy and reliability of PCR test results by verifying that the assay performs correctly. The controls typically include both positive and negative samples, which are crucial for validating the test's ability to detect the presence or absence of RSV genetic material.
Content Description
- Positive Control:
- Contains a known quantity of RSV genetic material.
- Used to confirm that the PCR assay can successfully amplify and detect RSV RNA.
- Ensures the PCR reagents and thermal cycler are functioning correctly.
- Negative Control:
- Contains no RSV genetic material.
- Used to verify that there is no contamination in the PCR reagents or sample preparation process.
- Ensures specificity of the PCR assay, confirming that non-target sequences are not amplified.
- Stability and Storage:
- Controls are typically lyophilized for long-term stability.
- Require reconstitution with nuclease-free water before use.
- Should be stored at recommended temperatures, often -20°C or lower, to maintain integrity.
- Usage Instructions:
- Controls should be processed and run in parallel with patient samples.
- Follow the same PCR protocol as used for test samples, including RNA extraction and amplification steps.
- Interpret results according to the assay's guidelines: the positive control should yield a positive result, and the negative control should yield a negative result.
- Quality Assurance:
- Regular use of controls helps identify potential issues with PCR assay components or procedures.
- Provides confidence in the test results, supporting diagnostic accuracy and patient care.
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