MonkeyPox PCR Controls

PCR controls for Monkeypox virus (MPXV) assays are critical components in molecular diagnostics that ensure accurate detection and quantification of MPXV DNA. Given the public health significance of Monkeypox outbreaks, integrating well-designed controls into PCR assays helps confirm assay performance, validate results, and prevent diagnostic errors. These controls monitor assay sensitivity, specificity, and integrity from sample preparation through amplification, providing confidence in test outcomes and compliance with quality standards.

Content:

• Data Interpretation:
  • Cycle Threshold (Ct) Values:
    • In real-time PCR assays, the Ct value indicates the cycle at which fluorescence surpasses the detection threshold.
• • Amplification Curves:
    • Exponential amplification curves corresponding to the target region indicate successful amplification of Monkeypox DNA in positive controls and patient samples.
  • Melt Curve Analysis (if applicable):
    • For SYBR Green-based assays, melt curve analysis can confirm the specificity of the amplified product by revealing its distinct melting temperature.

Application:

• Clinical Diagnostics:
  • Monkeypox PCR controls are used to confirm the presence of MPXV in clinical specimens such as lesion swabs, blood, or respiratory samples.
• Epidemiological Surveillance:
  • In public health laboratories, MPXV PCR controls ensure the consistency and reliability of surveillance data.
• Research and Assay Development:
  • During the development and validation of new Monkeypox PCR assays, controls assist in optimizing primer/probe design, determining assay sensitivity, and establishing limits of detection.

PCR controls for Monkeypox virus detection are integral to the accuracy, reliability, and safety of molecular diagnostic assays. By employing positive, negative, and internal controls, laboratories can validate every step of the PCR process, minimize the risk of false results, and ensure precise detection of MPXV.