CT PCR Controls

CT PCR Controls

Chlamydia trachomatis PCR controls are essential components of molecular assays designed to detect the presence of CT DNA in clinical samples. These controls are integrated into polymerase chain reaction (PCR) workflows to ensure accurate, reproducible results and to minimize errors such as false negatives or false positives. By validating each step of the process—from sample collection and nucleic acid extraction to amplification and detection—CT PCR controls uphold the assay's reliability and diagnostic integrity.

Content of CT PCR Controls

  • Positive Controls:
    • Contain a known quantity of CT DNA or synthetic DNA fragments that include target gene sequences specific to C. trachomatis, such as segments of the cryptic plasmid or major outer membrane protein (ompA) gene.
    • Serve to confirm that PCR reagents, primers, and probes are functioning correctly and that the assay can reliably detect CT DNA.
  • Negative Controls:
    • Consist of all PCR reagents except for the CT target DNA.
    • Often use a reaction mix with a sample matrix free of CT genetic material.

Application of CT PCR Controls

  • Assay Validation and Calibration:
    • During assay development and validation, positive controls are used to determine key performance characteristics such as sensitivity, specificity, linearity, and reproducibility.
    • Serial dilutions of the positive control can generate a standard curve in quantitative PCR (qPCR), which is critical for accurately measuring bacterial load in clinical specimens.
  • Quality Assurance in Routine Testing:
    • Each PCR run includes both positive and negative controls to monitor performance consistency and reagent integrity.
    • Regular use of these controls verifies that the assay is performing as expected, and that results obtained from patient samples are accurate, reducing the risk of misdiagnosis.

CT PCR controls—including positive, negative, internal, and extraction controls—are fundamental to the accuracy, reliability, and standardization of Chlamydia trachomatis PCR assays. 

494.00 494.0 USD
478.00 478.0 USD
494.00 494.0 USD