Precision in Clostridium PCR Testing: Leveraging AffiCHECK for Enhanced Control

Clostridium species are bacteria commonly found in the environment, some of which can cause severe infections in humans, such as Clostridium difficile-associated diarrhea. Polymerase Chain Reaction (PCR) is a valuable tool for detecting Clostridium species in clinical samples, but ensuring the accuracy of PCR results requires proper controls. AffiCHECK is a specialized control designed to enhance the reliability and validity of Clostridium PCR testing.

Understanding Clostridium PCR Controls

PCR controls are essential elements of molecular diagnostic testing, used to validate the accuracy and reliability of test results. These controls typically include positive controls, negative controls, and internal controls.

Positive controls contain known amounts of the target nucleic acid sequence, allowing laboratories to confirm that the PCR reaction is functioning correctly and detecting the specific target—in this case, Clostridium genetic material.

Negative controls contain no target nucleic acid sequence and are used to monitor for contamination or false positives. They ensure that the PCR reaction is not producing erroneous results due to contamination or other issues.

Internal controls are included in each PCR reaction and serve as indicators of the overall performance of the assay. They help detect potential inhibitors or other factors that may affect the efficiency of the PCR reaction.

Utilizing AffiCHECK for Clostridium PCR Testing

AffiCHECK is a quality control product specifically designed for Clostridium PCR testing. It comprises synthetic DNA fragments that mimic the genetic material of Clostridium species, serving as positive controls for PCR assays. These synthetic DNA fragments closely resemble the actual Clostridium DNA, allowing laboratories to validate the performance of their PCR tests accurately.

Key Features of AffiCHECK

Accurate Representation: AffiCHECK DNA fragments accurately mimic the genetic material of Clostridium species, providing a reliable positive control for PCR assays.


AffiCHECK is designed for long-term stability, ensuring consistent performance over time. This stability is crucial for maintaining the integrity of PCR testing processes.


AffiCHECK can be used with various PCR testing platforms and protocols, making it adaptable to different laboratory setups and workflows.

Quality Assurance

By incorporating AffiCHECK into their testing procedures, laboratories can enhance the quality assurance process and ensure the reliability of their Clostridium PCR test results.

Accurate detection of Clostridium species is critical for diagnosing and managing infections in clinical settings. AffiCHECK serves as a valuable tool for ensuring the accuracy and reliability of Clostridium PCR testing. By providing laboratories with a reliable positive control, AffiCHECK enhances the quality assurance process and contributes to the effective diagnosis and treatment of Clostridium infections.

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