Monkeypox, an orthopoxvirus, has emerged as a significant zoonotic disease. The diagnostic accuracy for Monkeypox is critical for effective management and containment. AffiCHECK is a novel diagnostic tool that has been employed to enhance the detection and confirmatory testing of Monkeypox virus. This article explores the technical details of AffiCHECK’s methodology and its application in Monkeypox diagnostics
Monkeypox, caused by the Monkeypox virus (MPXV), is a rare viral disease with symptoms similar to smallpox. Accurate diagnosis is essential for public health responses. Traditional diagnostic methods include PCR, ELISA, and viral culture, but these methods have limitations regarding specificity, sensitivity, and ease of use. AffiCHECK represents a new approach designed to address these limitations.
AffiCHECK Technology
AffiCHECK utilizes an advanced immunoassay technology that combines high-affinity antibodies with a rapid diagnostic platform. The assay is designed to detect specific antigens or antibodies associated with MPXV in clinical samples.
- Antibody-Antigen Interaction: AffiCHECK employs monoclonal antibodies that are specific to MPXV antigens. These antibodies are immobilized on a test strip. When a sample containing MPXV antigens is introduced, the antigens bind to the immobilized antibodies, forming an antigen-antibody complex.
- Detection Mechanism: The antigen-antibody complex is then detected using a colorimetric or fluorescence-based reporter system. The presence of a visible signal indicates a positive result for MPXV.
- Sensitivity and Specificity: AffiCHECK has been engineered to have high sensitivity and specificity for MPXV. The assay is capable of detecting low concentrations of MPXV antigens, which is crucial for early diagnosis. Cross-reactivity with other poxviruses is minimized through the use of highly specific antibodies.
Methodology
- Sample Collection: Clinical samples such as blood, skin lesions, or throat swabs are collected. These samples are processed and prepared for testing according to standard protocols.
- Assay Procedure: The prepared sample is added to the AffiCHECK test device. The device contains a nitrocellulose membrane with pre-coated antibodies specific to MPXV. The sample migrates through the membrane, and if MPXV antigens are present, they bind to the antibodies, forming a visible line.
- Result Interpretation: Results are interpreted based on the presence or absence of the detection line. A control line is also present to confirm that the assay has been performed correctly.
Advantages of AffiCHECK
- Rapid Turnaround: The AffiCHECK assay provides results within 15-30 minutes, allowing for quick decision-making.
- Ease of Use: The test is user-friendly and requires minimal training to perform, making it suitable for use in various healthcare settings.
- Cost-Effectiveness: Compared to traditional diagnostic methods, AffiCHECK offers a cost-effective solution for large-scale screening.
AffiCHECK represents a significant advancement in the rapid and accurate detection of Monkeypox virus. Its high sensitivity and specificity, coupled with ease of use, make it a valuable tool in the fight against Monkeypox. Further studies and field evaluations will be essential to fully establish its efficacy and potential applications in diverse settings.